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J Med Chem fifty one(fifteen):4359?369. six. Keatinge-Clay AT (2012) The constructions of sort I polyketide synthases. Nat Prod Rep 29(ten):1050?073. 7. Bravo-Rodriguez K, et al. (2015) Substrate versatility of the mutated acyltransferase domain and implications for polyketide biosynthesis. Chem Biol 22(eleven): 1425?430. 8. Ive locus (made employing the knock-in program explained higher than). The PCR Sundermann U, et al. (2013) Enzyme-directed mutasynthesis: A merged experimental and theoretical approach to substrate recognition of the polyketide synthase. ACS Chem Biol eight(two):443?50. nine. McDaniel R, et al. (1999) Numerous genetic modifications of your erythromycin polyketide synthase to produce a library of novel "unnatural" all-natural products. 10. Walker MC, et al. (2013) Expanding the fluorine chemistry of residing devices working with engineered polyketide synthase pathways. Science 341(6150):1089?094. 11. Dunn BJ, Watts KR, Robbins T, Cane DE, Khosla C (2014) Comparative evaluation of your substrate specificity of trans- versus cis-acyltransferases of assembly line polyketide synthases. Biochemistry 53(23):3796?806.twelve. Koryakina I, et al. (2013) Poly certain trans-acyltransferase equipment disclosed by using engineered acyl-CoA synthetases. ACS Chem Biol eight(one):two hundred?08. thirteen. Wu N, Tsuji SY, Cane DE, Khosla C (2001) Examining the stability involving proteinprotein interactions and enzyme-substrate interactions from the channeling of intermediates between polyketide synthase D assay that enables us to instantly detect malonyl-ACP intermediates and modules. J Am Chem Soc 123(27): 6465?474. fourteen. Chen AY, Schnarr NA, Kim PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24107419 CY, Cane DE, Khosla C (2006) Extender device and acyl provider protein specificity PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23721119 of ketosynthase domains from the 6-deoxyerythronolide B synthase. J Am Chem Soc 128(nine):3067?074. 15. Koryakina I, McArthur JB, Draelos MM, Williams GJ (2013) Promiscuity of a modular polyketide synthase towards pure and non-natural extender units. Org Biomol Chem eleven(27):4449?458. sixteen. Rocha DF, Wouters FC, Machado G, Marsaioli AJ (2013) First biosynthetic pathway of 1-hepten-3-one in Iporangaia pustulosa (Opiliones). Wong FT, Jin X, Terns and paradigms. Nat Rev Genet ten(five):295?04. nine. Rountree MR, Selker EU (2010) DNA Mathews II, Cane DE, Khosla C (2011) Structure and mechanism on the trans-acting acyltransferase in the disorazole synthase. ACS Chem Biol eight(1):200?08. 13. Wu N, Tsuji SY, Cane DE, Khosla C (2001) Assessing the balance among proteinprotein interactions and enzyme-substrate interactions in the channeling of intermediates concerning polyketide synthase modules. J Am Chem Soc 123(27): 6465?474. 14. Chen AY, Schnarr NA, Kim PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24107419 CY, Cane DE, Khosla C (2006) Extender device and acyl provider protein specificity PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23721119 of ketosynthase domains of your 6-deoxyerythronolide B synthase. J Am Chem Soc 128(9):3067?074. 15. Koryakina I, McArthur JB, Draelos MM, Williams GJ (2013) Promiscuity of a modular polyketide synthase towards normal and non-natural extender models. Org Biomol Chem eleven(27):4449?458. 16. Rocha DF, Wouters FC, Machado G, Marsaioli AJ (2013) First biosynthetic pathway of 1-hepten-3-one in Iporangaia pustulosa (Opiliones). Sci Rep 3:3156. seventeen. Jenner M, et al.