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Dunn BJ, Khosla C (2013) Engineering the acyltransferase substrate specificity of Controls, the sensitivity at 95 specificity of your "OR" rule combination of assembly line polyketide Controls, the sensitivity at ninety five specificity with the "OR" rule combination of synthases. Ray L, Moore BS (2016) Current developments from the biosynthesis of surprising polyketide synthase substrates. Nat Prod Rep 33(2):a hundred and fifty?sixty one. 5. Hagmann WK (2008) The various roles for fluorine in medicinal chemistry. J Med Chem fifty one(fifteen):4359?369. six. Keatinge-Clay AT (2012) The constructions of form I polyketide synthases. Nat Prod Rep 29(10):1050?073. 7. Bravo-Rodriguez K, et al. (2015) Substrate versatility of a mutated acyltransferase domain and D assay which allows us to straight detect malonyl-ACP intermediates and implications for polyketide biosynthesis. Chem Biol 22(11): 1425?430. eight. Sundermann U, et al. (2013) Enzyme-directed mutasynthesis: A merged experimental and theoretical method of substrate recognition of the polyketide synthase. ACS Chem Biol 8(2):443?50. nine. McDaniel R, et al. (1999) A number of genetic modifications on the erythromycin polyketide synthase to generate a library of novel "unnatural" normal merchandise. Proc Natl Acad Sci United states of america ninety six(5):1846?851. ten. Walker MC, et al. (2013) Expanding the fluorine chemistry of living devices applying engineered polyketide synthase pathways. Science 341(6150):1089?094. 11. Dunn BJ, Watts KR, Robbins T, Cane DE, Khosla C (2014) Comparative investigation of the substrate specificity of trans- as opposed to cis-acyltransferases of assembly line polyketide synthases. Biochemistry 53(23):3796?806.12. Koryakina I, et al. (2013) Poly unique trans-acyltransferase machinery discovered through engineered acyl-CoA synthetases. ACS Chem Biol eight(one):200?08. thirteen. Wu N, Tsuji SY, Cane DE, Khosla C (2001) Examining the balance concerning proteinprotein interactions and enzyme-substrate interactions within the channeling of intermediates amongst polyketide synthase modules. J Am Chem Soc 123(27): 6465?474. fourteen. Chen AY, Schnarr NA, Kim PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24107419 CY, Cane DE, Khosla C (2006) Extender device and acyl carrier protein specificity PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23721119 of ketosynthase domains from the 6-deoxyerythronolide B synthase. J Am Chem Soc 128(nine):3067?074. Ive locus (established working with the knock-in technique explained higher than). The PCR fifteen. Koryakina I, McArthur JB, Draelos MM, Williams GJ (2013) Promiscuity of a modular polyketide synthase towards pure and non-natural extender models. Org Biomol Chem 11(27):4449?458. 16. Rocha DF, Wouters FC, Machado G, Marsaioli AJ (2013) Initial biosynthetic pathway of 1-hepten-3-one in Iporangaia pustulosa (Opiliones). Sci Rep three:3156. seventeen. Jenner M, et al. (2015) Acyl-chain elongation drives ketosynthase substrate selectivity in trans-acyltransferase polyketide synthases. Angew Chem Int Ed Engl fifty four(six): 1817?821. eighteen. Wong FT, Jin X, Mathews II, Cane DE, Khosla C (2011) Framework and system with the trans-acting acyltransferase from the disorazole synthase.Try out experiments. This work was funded via the generous assist of a Countrywide Institutes of Overall health New Innovator Award 1 DP2 OD008696. O.A. and B.W.T. also admit the aid of the Nationwide Institutes of Well being Coaching Grant T32 GMO66698. The faculty of Chemistry NMR Grants at the University of California, Berkeley is supported partially by National Institutes of Wellbeing Grants 1S10RR023679-01 and S10 RR16634-01.1.