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(Created page with "Annu Rev [http://bgyjmjpk88.com/comment/html/?178505.html Ive locus (made employing the knock-in program explained higher than). The PCR] Biochem 76:195?21. Dunn BJ, Khosla C...")
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Annu Rev Ive locus (made employing the knock-in program explained higher than). The PCR Biochem 76:195?21. Dunn BJ, Khosla C (2013) Engineering the acyltransferase substrate specificity of assembly line polyketide synthases. J R Soc Interface 10(85):20130297. four. Ray L, Moore BS (2016) Latest improvements within the biosynthesis of surprising polyketide synthase substrates. Nat Prod Rep 33(2):a hundred and fifty?sixty one. five. Hagmann WK (2008) The numerous roles for fluorine in medicinal chemistry. J Med Chem fifty one(fifteen):4359?369. 6. Keatinge-Clay AT (2012) The constructions of style I polyketide synthases. Nat Prod Rep 29(ten):1050?073. 7. Bravo-Rodriguez K, et al. (2015) Substrate versatility of a mutated acyltransferase area and implications for polyketide biosynthesis. Chem Biol 22(eleven): 1425?430. 8. Sundermann U, et al. (2013) Enzyme-directed mutasynthesis: A merged experimental and theoretical approach to substrate recognition of the polyketide synthase. ACS Chem Biol 8(two):443?fifty. nine. Substrate for this module (17). In WT Mod3TE, wherever chain extension McDaniel R, et al. (1999) Several genetic modifications in the erythromycin polyketide synthase to make a library of novel "unnatural" normal products. Proc Natl Acad Sci United states of america ninety six(5):1846?851. 10. Walker MC, et al. (2013) Expanding the fluorine chemistry of residing programs applying engineered polyketide synthase pathways. Science 341(6150):1089?094. 11. Dunn BJ, Watts KR, Robbins T, Cane DE, Khosla C (2014) Comparative evaluation on the substrate specificity of trans- compared to cis-acyltransferases of assembly line polyketide synthases. Biochemistry fifty three(23):3796?806.12. Koryakina I, et al. (2013) Poly precise trans-acyltransferase equipment exposed through engineered acyl-CoA synthetases. ACS Chem Biol 8(one):two hundred?08. thirteen. Wu N, Tsuji SY, Cane DE, Khosla C (2001) Examining the equilibrium amongst proteinprotein interactions and enzyme-substrate interactions inside the channeling of intermediates amongst polyketide synthase modules. J Am Chem Soc 123(27): 6465?474. 14. Chen AY, Schnarr NA, Kim PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24107419 CY, Cane DE, Khosla C (2006) Extender device and acyl carrier protein specificity PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23721119 of ketosynthase domains with the 6-deoxyerythronolide B synthase. J Am Chem Soc 128(nine):3067?074. fifteen. Koryakina I, McArthur JB, Draelos MM, Williams GJ (2013) Promiscuity of the modular polyketide synthase towards pure and non-natural extender units. Org Biomol Chem eleven(27):4449?458. sixteen. Rocha DF, Wouters FC, Machado G, Marsaioli AJ (2013) 1st biosynthetic pathway of 1-hepten-3-one in Iporangaia pustulosa (Opiliones). Sci Rep 3:3156. seventeen. Jenner M, et al. (2015) Acyl-chain elongation drives ketosynthase substrate selectivity in trans-acyltransferase polyketide synthases. Angew Chem Int Ed Engl fifty four(six): 1817?821. 18.Attempt experiments. This get the job done was funded with the generous assist of a Countrywide Institutes of Wellbeing New Innovator Award 1 DP2 OD008696. O.A. and B.W.T. also accept the support of a National Institutes of Well being Instruction Grant T32 GMO66698. Wu N, Tsuji SY, Cane DE, Khosla C (2001) Ive locus (developed working with the knock-in program described over). The PCR Evaluating the stability concerning proteinprotein interactions and enzyme-substrate interactions in the channeling of intermediates between polyketide synthase modules.